doi: 10.1093/nar/gks808, Kobayashi, M., Izui, H., Nagasawa, T., and Yamada, H. (1993). Nucleic Acids Res. Aliphatic nitrilases were derived from Comamonas testosteroni (AAA82085) (Lévy-Schil et al., 1995), Synechocystis sp. The tree with the highest log likelihood (−31012.19) is shown. In case of no growth a 0 was given, whereas similar growth compared to the control was treated as 4. The nitrogenous bases of this invention are tri-substituted at the nitrogen atom and comprise heterocyclic nitrogen compounds such as pyridine and tri-substituted amines. doi: 10.1007/s12600-017-0629-3, Marhan, J. doi: 10.1038/nmeth.f.303. Polar Biol. The amino acid sequence of the aliphatic amidase from Pseudomonas aeruginosa. 57, 9–14. Appl. doi: 10.1016/0278-6915(88)90111-1, Wibberg, D., Luque-Almagro, V. M., Igeño, M. I., Bremges, A., Roldán, M. D., Merchán, F., et al. presents a host of problems requiring special equipment, sophisticated techniques, and constant attention. Appl. 69, 4359–4366. doi: 10.1016/j.jbiotec.2014.02.004, Willhite, C. C., and Smith, R. P. (1981). Lett. 60, 459–481. J. Biochem. (1997). MTCC 10674 and its application in the synthesis of α-hydroxyisobutyric acid. Sci. doi: 10.1128/jb.172.9.4807-4815.1990, Kobayashi, M., Yanaka, N., Nagasawa, T., and Yamada, H. (1992b). Pre-cultures of these strains were grown in LB medium at 30°C and 180 rpm (Multitron shaker, Infors, Einsbach, Germany) for 24 h. Afterwards, each strain was plated on a quadrant of a LB or M9-HEPES plate containing one of the nine nitriles or the respective carboxylic acid in a concentration of 5, 10, 15, 20, and 25 mM. Nitrilase in biosynthesis of the plant hormone indole-3-acetic acid from indole-3-acetonitrile: cloning of the Alcaligenes gene and site-directed mutagenesis of cysteine residues. 175, 67–68. PCR reactions for each culture were performed in triplicate, pooled in equal amounts, and purified with the NucleoMag 96 PCR kit (Macherey-Nagel, Düren, Germany) according to the manufacturer's protocol. Nucleic Acids Res. Toxicol. The new cultures were adjusted to an OD600 of 0.1 and incubated again at 25°C and 75 rpm. A process for producing p-tert-butyl-benzonitrile from p-tert-butyl benzoic acid, said process comprising in combination: a. forming a complex of said p-tert-butyl-benzoic acid with pyridine. Inhibition of the Agrobacterium tumefaciens TraR quorum-sensing regulator. 69, 4794–4805. Microbiol. The carboxylic acid is thereby freed for reaction with the ammonia. doi: 10.1021/ar00022a001, Schreiner, U., Hecher, B., Obrowsky, S., Waich, K., Klempier, N., Steinkellner, G., et al. Thus, this invention teaches production of nitriles from carboxylic acids using a carboxylic acid-nitrogenous base complex as the raw material. Life Sci. It is preferred that the boiling point of the nitrogenous base be low compared to that of the resulting nitrile so that it can be readily separated and recovered by distillation of the reactor effluent. For amplification of the V3-V4 region of bacterial 16S rRNA genes, the primers S-D-Bact-0341-b-S-17 and S-D-Bact-0785-a-A-21 (Klindworth et al., 2013) were used. Pollut. In most cases, the complexes of the invention are liquids and are thus easily handled. The carboxylic acid derivatives along can be hydrolyzed to produce carboxylic acids. c. recovering the p-tert-butyl-benzonitrile thus produced. Curr. Mixtures of the above mentioned compounds are also suitable for forming complexes. doi: 10.1016/0378-1119(95)00242-X, Lewis, D. F. V., and Wiseman, A. FEBS Lett. Effects of growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria. 172, 4807–4815.

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