To achieve these goals, we frequently collaborate with chromatography and MS companies to test newest column materials, instrumentation and software. © IMBA - Institute of Molecular Biotechnology GmbH. Despite the tremendous technological improvements in ultrasensitive proteomics workflows and instrumentation, the comprehensive characterization of individual mammalian cells still challenges the sensitivity of currently available tandem mass-spectrometry-based proteome analysis methods. Driven by the rapid development of instrumentation, analysis methods and computing tools, MS based proteomics is at the forefront of techniques in modern life science research and nowadays allows the study of nearly complete proteomes. IMBA - Institute of Molecular Biotechnology. Current MS instruments produce vast amounts of data. For quality control, we developed SimpatiQCo (Pichler et al., J Proteome Res. 1030 Vienna, Austria. 2014), ptmRS (Taus et al, J Proteome Res. Commun. We have set up methods for cross-linking protein complexes with a low number of subunits/binding partners by different cross-linkers including an enrichment procedure (Rampler et al., J Proteome Res 2015, Matzinger et al., J. Proteome Res. 2020). 2012), which allows user-friendly monitoring of key parameters of all LC-MS platforms to assure reproducible and stable performance of the systems at the highest possible sensitivity. Furthermore, we investigate alternative labeling and acquisition strategies to enable accurate single cell or other very low input proteome measurements. In the Mechtler lab, we are interested in developing new methods to increase sensitivity in protein identification, accuracy and precision of protein quantification as well as for the detection of posttranslational modifications. 2019) and Hyperplex for quantification of label-free and isobarically labelled samples. Gradually over the past two decades, mass spectrometers were interfaced with a number of protein chemistry assays to create detectors providing superior information. In a cross-linking experiment, a reagent forms covalent bonds between specific residues that are in close spatial proximity to each other, thus revealing distance restraints between residues and hence interaction sites either within a protein or between different proteins. By using our website, you agree to it. Another focus is the development of new algorithms for MS data interpretation and bioinformatic analysis. With … MS has become invaluable across a broad range of fields and applications, including proteomics.

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